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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM126A Polyclonal antibody (20759-1-AP)

    WB analysis using 20759-1-AP (same clone as 20759-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20759-1-AP (TMEM126A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM126A Polyclonal antibody (20759-1-AP)

    IF Staining of HeLa using 20759-1-AP (same clone as 20759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (20759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM126A Polyclonal antibody (20759-1-AP)

    IF Staining of HeLa using 20759-1-AP (same clone as 20759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (20759-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20759-1-PBS in a different storage buffer formulation.

TMEM126A Polyclonal antibody, PBS Only

TMEM126A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20759-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

OPA7, TMEM, TMEM126

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20759-1-PBS targets TMEM126A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14705

Product name: Recombinant human TMEM126A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 67-195 aa of BC007875

Sequence: AGLPMAGIPFLTTDLTYRCFVSFPLNTGDLDCETCTITRSGLTGLVIGGLYPVFLAIPVNGGLAARYQSALLPHKGNILSYWIRTSKPVFRKMLFPILLQTMFSAYLGSEQYKLLIKALQLSEPGKEIH

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 126A
Calculated molecular weight 195 aa, 22 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC007875
Gene Symbol TMEM126A
Gene ID (NCBI) 84233
RRIDAB_3669345
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H061
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.

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