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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using TMEM115 Polyclonal antibody (25536-1-AP)

    WB analysis of U2OS using 25536-1-AP (same clone as 25536-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM115 Polyclonal antibody (25536-1-AP)

    WB analysis using 25536-1-AP (same clone as 25536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human ovary cancer using 25536-1-AP (same clone as 25536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human ovary cancer using 25536-1-AP (same clone as 25536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP (same clone as 25536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TMEM115 Polyclonal antibody (25536-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP (same clone as 25536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25536-1-PBS in a different storage buffer formulation.

TMEM115 Polyclonal antibody, PBS Only

TMEM115 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25536-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Transmembrane protein 115, Placental protein 6, PL6, LUCA11.2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25536-1-PBS targets TMEM115 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22258

Product name: Recombinant human TMEM115 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 233-351 aa of BC017367

Sequence: QPVVGLLANLVHSLLVKVKICQKTVKRYDVGAPSSITISLPGTDPQDAERRRQLALKALNERLKRVEDQSIWPSMDDDEEESGAKVDSPLPSDKAPTPPGKGAAPESSLITFEAAPPTL

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 115
Calculated molecular weight 351 aa, 38 kDa
Observed molecular weight35-38 kDa
GenBank accession numberBC017367
Gene Symbol TMEM115
Gene ID (NCBI) 11070
RRIDAB_3669482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12893
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM115 also known as PL6, located on chromosome 3p21.3, was originally thought to function as a tumor suppressor. TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.

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