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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Retrolinkin Polyclonal antibody (15177-1-AP)

    WB analysis using 15177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15177-1-AP (Retrolinkin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Retrolinkin Polyclonal antibody (15177-1-AP)

    WB analysis of HeLa using 15177-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 15177-1-AP (Retrolinkin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Retrolinkin Polyclonal antibody (15177-1-AP)

    IHC staining of mouse brain using 15177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15177-1-AP (Retrolinkin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Retrolinkin Polyclonal antibody (15177-1-AP)

    IHC staining of mouse brain using 15177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15177-1-AP (Retrolinkin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Retrolinkin Polyclonal antibody

Retrolinkin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15177-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

TMEM108, UNQ1875/PRO4318, Transmembrane protein 108, RTLN, KIAA1690

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15177-1-AP targets Retrolinkin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Retrolinkin fusion protein Ag7076 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 108
Calculated molecular weight 60 kDa
Observed molecular weight 55-60 kDa and 65-70 kDa
GenBank accession numberBC000568
Gene Symbol TMEM108
Gene ID (NCBI) 66000
RRIDAB_2204598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retrolinkin, also named as TMEM108, KIAA1690 and RTLN, is a membrane protein which plays an important role in retrograde axonal transport. It's highly enriched in neuronal endosomes, binds directly to BPAG1n4. This gene has two isoforms with calculated MW 60 kDa and 50 kDa. With modification, the MW of these two forms will be migrated to 65-70 kDa and 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for Retrolinkin antibody 15177-1-APDownload protocol
IHC protocol for Retrolinkin antibody 15177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols