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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM106B (1-46aa) Monoclonal antibody (60333-1-Ig)

    WB analysis using 60333-1-Ig (same clone as 60333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMEM106B (1-46aa) Monoclonal antibody (60333-1-Ig)

    WB analysis of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

    WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMEM106B (1-46aa) Monoclonal antibody (60333-1-Ig)

    WB analysis of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM106B (1-46aa) Monoclonal antibody (60333-1-Ig)

    WB analysis using 60333-1-Ig (same clone as 60333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM106B (1-46aa) Monoclonal antibody (60333-1-Ig)

    IP experiment of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

    IP result of anti-TMEM106B (IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM106B Monoclonal antibody, PBS Only

TMEM106B Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 60333-1-PBS
Clone No.5D1F8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

TMEM106B (1-46aa), 5D1F8, Transmembrane protein 106B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60333-1-PBS targets TMEM106B in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21448

Product name: Recombinant human TMEM106B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-46 aa of BC033901

Sequence: MGKSLSHLPLHSSKEDAYDGVTSENMRNGLVNSEVHNEDGRNGDVS

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 106B
Calculated molecular weight 31 kDa
Observed molecular weight ~40 kDa
GenBank accession numberBC033901
Gene Symbol TMEM106B
Gene ID (NCBI) 54664
RRIDAB_2881442
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NUM4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

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