Validation Data Gallery
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Tested Applications
| Positive IHC detected in | mouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22272 Product name: Recombinant human TMEM100 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of BC010128 Sequence: MTEEPIKEILGAPKAHMAATMEKSPKSEVVITTVPLVSEIQLMAATGGTELSCYRCIIPFAVVVFIAGIVVTAVAYSFNSHGSIISIFGLVVLSSGLFLLASSALCWKVRQRSKKAKRRESQTALVANQRSLFA 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 100 |
| Calculated molecular weight | 134 aa, 14 kDa |
| GenBank accession number | BC010128 |
| Gene Symbol | TMEM100 |
| Gene ID (NCBI) | 55273 |
| RRID | AB_2880138 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NV29 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM100 antibody 25581-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomolecules Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods | ||
World J Clin Oncol Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma | ||
Front Cell Neurosci A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells |