×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TMED9 Recombinant monoclonal antibody (86802-1-RR)

    WB analysis using 86802-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86802-1-RR (TMED9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMED9 Recombinant monoclonal antibody (86802-1-RR)

    IF Staining of A549 using 86802-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TMED9 antibody (86802-1-RR, Clone: 251396A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TMED9 Recombinant monoclonal antibody (86802-1-RR)

    IF Staining of MCF-7 using 86802-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TMED9 antibody (86802-1-RR, Clone: 251396A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TMED9 Recombinant monoclonal antibody

TMED9 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86802-1-RR
Clone No.251396A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells, MCF-7 cells, OVCAR-3 cells, A2780 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86802-1-RR targets TMED9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13813

Product name: Recombinant human TMED9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC001123

Sequence: MAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH

相同性解析による交差性が予測される生物種
Full Name transmembrane emp24 protein transport domain containing 9
Calculated molecular weight 235 aa, 27 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC001123
Gene Symbol TMED9
Gene ID (NCBI) 54732
RRIDAB_3745128
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BVK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

Protocols

Product Specific Protocols
IF protocol for TMED9 antibody 86802-1-RRDownload protocol
WB protocol for TMED9 antibody 86802-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...