Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21620-1-AP (same clone as 21620-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 21620-1-AP (same clone as 21620-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
WB analysis of pig liver using 21620-1-AP (same clone as 21620-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 21620-1-AP (same clone as 21620-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 21620-1-AP (same clone as 21620-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
WB analysis using 21620-1-AP (same clone as 21620-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)
IP result of anti-TMED9 (IP:21620-1-AP, 4ug; Detection:21620-1-AP 1:4000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 21620-1-AP (TMED9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 21620-1-AP (same clone as 21620-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TMED9 Polyclonal antibody, PBS Only

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21620-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Glycoprotein 25L2, GMP25, GP25L2, p24 family protein alpha-2, p24alpha2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21620-1-PBS targets TMED9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13813 Product name: Recombinant human TMED9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC001123 Sequence: MAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH 相同性解析による交差性が予測される生物種
Full Name	transmembrane emp24 protein transport domain containing 9
Calculated molecular weight	235 aa, 27 kDa
Observed molecular weight	24-27 kDa
GenBank accession number	BC001123
Gene Symbol	TMED9
Gene ID (NCBI)	54732
RRID	AB_10858623
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BVK6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

WB analysis of mouse pancreas using 21620-1-AP (same clone as 21620-1-PBS)

WB analysis of pig liver using 21620-1-AP (same clone as 21620-1-PBS)

WB analysis of mouse brain using 21620-1-AP (same clone as 21620-1-PBS)

WB analysis of mouse lung using 21620-1-AP (same clone as 21620-1-PBS)

WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

IP experiment of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

IF Staining of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

IF Staining of HEK-293 using 21620-1-AP (same clone as 21620-1-PBS)

TMED9 Polyclonal antibody, PBS Only

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™