• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

TMED4 Polyclonal antibody

TMED4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14141-1-AP

Synonyms

ERS25, HNLF, TMED4

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 14141-1-AP

    WB analysis of mouse liver using 14141-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14141-1-AP

    WB analysis of mouse liver using 14141-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14141-1-AP

    WB analysis of HepG2 using 14141-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14141-1-AP

    WB analysis of mouse liver using 14141-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 14141-1-AP

    IP experiment of mouse liver using 14141-1-AP

    IP result of anti-TMED4 (IP:14141-1-AP, 4ug; Detection:14141-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

  • IF Staining of HepG2 using 14141-1-AP

    IF Staining of HepG2 using 14141-1-AP

    Immunofluorescent analysis of HepG2 cells using 14141-1-AP (TMED4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14141-1-AP targets TMED4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMED4 fusion protein Ag5234 相同性解析による交差性が予測される生物種
Full Name transmembrane emp24 protein transport domain containing 4
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC057851
Gene symbol TMED4
Gene ID (NCBI) 222068
RRIDAB_10642696
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMED4 antibody 14141-1-APDownload protocol
IF protocol for TMED4 antibody 14141-1-APDownload protocol
IP protocol for TMED4 antibody 14141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated
View Article
humanWB

Ann Transl Med

Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer

Authors - Zheng Fang
  • KD Validated
View Article