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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMED1 Recombinant monoclonal antibody (87837-1-RR)

    WB analysis using 87837-1-RR (same clone as 87837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using TMED1 Recombinant monoclonal antibody (87837-1-RR)

    WB analysis of PC-12 using 87837-1-RR (same clone as 87837-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 87837-1-RR (TMED1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human lung cancer （Adenocarcinoma） using TMED1 Recombinant monoclonal antibody (87837-1-RR)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human lung cancer （Adenocarcinoma） using TMED1 Recombinant monoclonal antibody (87837-1-RR)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87837-1-RR (same clone as 87837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87837-1-RR (TMED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMED1 Recombinant monoclonal antibody (87837-1-RR)

    IF Staining of HeLa using 87837-1-RR (same clone as 87837-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMED1 antibody (87837-1-RR, Clone: 253497F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87837-1-PBS in a different storage buffer formulation.

  • Affinity of 87837-1-RR

    Affinity of 87837-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87837-1-RR against Human TMED1 were performed. The affinity constant is 34.2 pM.

TMED1 Recombinant monoclonal antibody, PBS Only

TMED1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87837-1-PBS
Clone No.253497F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

IL1RL1L, IL1RL1LG, Interleukin-1 receptor-like 1 ligand, p24 family protein gamma-1, p24gamma1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87837-1-PBS targets TMED1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5903

Product name: Recombinant human TMED1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-194 aa of NM_006858

Sequence: AGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDVDFTLESPQGVLLVSESRKADGVHTVEPTEAGDYKLCFDNSFSTISEKLVFFELIFDSLQDDEEVEGWAEAVEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEGNLERVN

相同性解析による交差性が予測される生物種
Full Name transmembrane emp24 protein transport domain containing 1
Calculated molecular weight25 kDa
Observed molecular weight30 kDa
GenBank accession numberNM_006858
Gene Symbol TMED1
Gene ID (NCBI) 11018
RRIDAB_3745778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13445
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transmembrane p24 trafficking protein 1 (TMED1) belongs to the TMED protein family, also known as the p24 family, and plays a part in protein vesicular trafficking. As the critical regulator of protein trafficking along the secretory pathway, the TMED proteins influence the composition, structure, and function of the endoplasmic reticulum and the Golgi apparatus. Disruption of the secretory pathway could affect cell differentiation and survival, ultimately resulting in disease.(PMID: 38392302)

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