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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMCO6 Polyclonal antibody

TMCO6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20117-1-AP

Synonyms

PRO1580, TMCO6

Validation Data Gallery

Applications:
  • WB analysis of human testis using 20117-1-AP

    WB analysis of human testis using 20117-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20117-1-AP (TMCO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 20117-1-AP

    WB analysis of mouse brain using 20117-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20117-1-AP (TMCO6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 20117-1-AP

    IHC staining of human spleen using 20117-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20117-1-AP (TMCO6 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 20117-1-AP

    IHC staining of human spleen using 20117-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20117-1-AP (TMCO6 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 20117-1-AP

    IHC staining of human lung using 20117-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20117-1-AP (TMCO6 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 20117-1-AP

    IHC staining of human lung using 20117-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20117-1-AP (TMCO6 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman testis tissue, mouse brain tissue
Positive IHC detected inhuman spleen tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20117-1-AP is TMCO6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMCO6 fusion protein Ag13992 相同性解析による交差性が予測される生物種
Full Name transmembrane and coiled-coil domains 6
Calculated molecular weight 493 aa, 54 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC001910
Gene symbol TMCO6
Gene ID (NCBI) 55374
RRIDAB_10733118
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMCO6 antibody 20117-1-APDownload protocol
IHC protocol for TMCO6 antibody 20117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols