Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, A431 cells, HepG2 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 4 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TM9SF4 fusion protein Ag22335 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane 9 superfamily protein member 4 |
| Calculated molecular weight | 642 aa, 75 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC021107 |
| Gene Symbol | TM9SF4 |
| Gene ID (NCBI) | 9777 |
| RRID | AB_2880149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92544 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
| IHC protocol for TM9SF4 antibody 25595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.
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Stem Cell Res Ther TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes
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J Exp Clin Cancer Res CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells. | ||
Cell Mol Gastroenterol Hepatol TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease
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Nat Commun TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis
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