Validation Data Gallery
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Tested Applications
| Positive WB detected in | human lung tissue, BxPC-3 cells, human heart tissue, human kidney tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
12033-1-AP targets TM7SF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2637 Product name: Recombinant human TM7SF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-418 aa of BC012857 Sequence: APVSALAPGGNSGNPIYDFFLGREVNPRICFFDFKYFCELRPGLIGWVLINLALLMKEAELRGSPSLAMWLVNGFQLLYVGDALWHEEAVLTTMDITHDGFGFMLAFGDMAWVPFTYSLQAQFLLHHPQPLGLPMASVICLINAIGYYIFRGANSQKNTFRKNPSDPRVAGLETISTATGRKLLVSGWWGMVRHPNYLGDLIMALAWSLPCGVSHLLPYFYLLYFTALLVHREARDERQCLQKYGLAWQEYCRRVPYRIMPYIY 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane 7 superfamily member 2 |
| Calculated molecular weight | 418 aa, 46 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC012857 |
| Gene Symbol | TM7SF2 |
| Gene ID (NCBI) | 7108 |
| RRID | AB_2203934 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| IHC protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| WB protocol for TM7SF2 antibody 12033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci METTL16 Promotes Lipid Metabolic Reprogramming and Colorectal Cancer Progression | ||
Phytomedicine Dingxin recipe Ⅲ ameliorates atherosclerosis through stard4-mediated regulation of hepatic lipid metabolism. | ||
J Ethnopharmacol Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer | ||
iScience TMEM97 regulates cholesterol biosynthesis and mitochondrial metabolism in gastric carcinoma. | ||
Curr Oncol TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis | ||
PLoS One Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis. |