Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27671-1-AP targets TM6SF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26527 Product name: Recombinant human TM6SF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 289-377 aa of NM_001001524 Sequence: MFPGCSWLPDWALVFAGGIGQAQFSHMGASMHLRTPFTYRVPEDTWGCFFVCNLLYALGPHLLAYRCLQWPAFFHQPPPSDPLALHKKQH 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane 6 superfamily member 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | NM_001001524 |
| Gene Symbol | TM6SF2 |
| Gene ID (NCBI) | 53345 |
| RRID | AB_3085983 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TM6SF2 is mainly expressed in the liver, intestine, kidney, and in other tissues at low levels. In terms of subcellular localization, TM6SF2 is localized in the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment, and Golgi of liver cells and enterocytes. The loss of TM6SF2 promotes the release of free fatty acids from IECs, leading to intestinal barrier dysfunction, gut microbiota dysbiosis and metabolite alterations (PMID: 29232562, 27013658). TM6SF2 encodes a protein predicted to have 7-10 transmembrane domains, containing 351 amino acids (39.5-kDa) with an estimated isoelectric point (pI) of 8.29 (PMID: 39779889).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TM6SF2 antibody 27671-1-AP | Download protocol |
| WB protocol for TM6SF2 antibody 27671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |