Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Jurkat cells, Ramos cells, SH-SY5Y cells, mouse colon tissue, rat colon tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27769-1-AP targets TLR9 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25615 Product name: Recombinant human TLR9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 925-1032 aa of BC032713 Sequence: ASVYGSRKTLFVLAHTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILSPDGRRSRYVRLRQRLCRQSVLLWPHQPSGQRSFWAQLGMALTRDNHHFYNRNFCQGPTAE 相同性解析による交差性が予測される生物種 |
| Full Name | toll-like receptor 9 |
| Calculated molecular weight | 1032 aa, 116 kDa |
| Observed molecular weight | 100-130 kDa, 60-80 kDa |
| GenBank accession number | BC032713 |
| Gene Symbol | TLR9 |
| Gene ID (NCBI) | 54106 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR96 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TLR9 is a member of the Toll-like receptor family which has been described in a wide-variety of animal species. TLR9 recognizes DNA motifs containing the dinucleotide CG (cytosine-guanine) where the C is unmethylated (CpG-containing DNA). TLR9 is expressed in a wide variety of cells such as B-cells, eosinophils, neutrophils, dendritic cells, macrophages, bronchial epithelial cells, and type-I and type-II lung cells, amongst others. Like all TLRs that recognize nucleic acids, TLR9 is confined to the endoplasmic reticulum and to endolysosomes. Western blot analysis revealed a 130 kDa band (entire form). 100 kDa fragment (soluble form) and a supplementary 60~80 kDa band (cleaved fragment) (PMID: 24582318,18820679, 21604257).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TLR9 antibody 27769-1-AP | Download protocol |
| WB protocol for TLR9 antibody 27769-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |