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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TLR2 Monoclonal antibody (66645-1-Ig)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using TLR2 Monoclonal antibody (66645-1-Ig)

    WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using TLR2 Monoclonal antibody (66645-1-Ig)

    WB analysis of U-937 using 66645-1-Ig (same clone as 66645-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TLR2 Monoclonal antibody (66645-1-Ig)

    IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TLR2 Monoclonal antibody (66645-1-Ig)

    IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50388-1

    Cytometric bead array standard curve of MP50388-1

    Cytometric bead array standard curve of MP50388-1, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-2-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50388-3

    Cytometric bead array standard curve of MP50388-3

    Cytometric bead array standard curve of MP50388-3, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-4-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO Validated

TLR2 Monoclonal antibody, PBS Only (Capture)

TLR2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66645-1-PBS
Clone No.1C1D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66645-1-PBS targets TLR2 as part of a matched antibody pair:

MP50388-1: 66645-1-PBS capture and 66645-2-PBS detection (validated in Cytometric bead array)

MP50388-3: 66645-1-PBS capture and 66645-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25626

Product name: Recombinant human TLR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 19-302 aa of BC033756

Sequence: KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDR

相同性解析による交差性が予測される生物種
Full Name toll-like receptor 2
Calculated molecular weight 784 aa, 90 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC033756
Gene Symbol TLR2
Gene ID (NCBI) 7097
RRIDAB_2882003
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60603
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

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