Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293T cells, MOLT-4 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85877-1-RR targets TKTL1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4913 Product name: Recombinant human TKTL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC025382 Sequence: MADAEARAEFPEEARPDRGTLQVFQDMASRLRIHSIRATCSTSSGHPTSCSSSSEIMSVLFFYIMRYKQSDPENPDNDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAFASYYSLDNLVATFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPTAVVAKTFKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSPPDYRVGDKIATRKACGLALA 相同性解析による交差性が予測される生物種 |
| Full Name | transketolase-like 1 |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC025382 |
| Gene Symbol | TKTL1 |
| Gene ID (NCBI) | 8277 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51854 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transketolase-like 1 (TKTL1) is a gene from the transketolase family that in fetal human neocortex is preferentially expressed in the two classes of neuroprogenitors, the apical progenitors in the ventricular zone and the basal progenitors in the subventricular zone.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TKTL1 antibody 85877-1-RR | Download protocol |
| WB protocol for TKTL1 antibody 85877-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |