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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis using 68495-1-Ig (same clone as 68495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TJAP1 Monoclonal antibody (68495-1-Ig)

    WB analysis of HepG2 using 68495-1-Ig (same clone as 68495-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68495-1-PBS in a different storage buffer formulation.

TJAP1 Monoclonal antibody, PBS Only

TJAP1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68495-1-PBS
Clone No.1H1F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

1H1F10, PILT, Tight junction-associated protein 1, TJAP 1, TJP4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68495-1-PBS targets TJAP1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11807

Product name: Recombinant human TJAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC064401

Sequence: MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALERELEIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNKLLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPSLAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGECHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISLS

相同性解析による交差性が予測される生物種
Full Name tight junction associated protein 1 (peripheral)
Calculated molecular weight 547 aa, 61 kDa
Observed molecular weight 86-95 kDa
GenBank accession numberBC064401
Gene Symbol TJAP1
Gene ID (NCBI) 93643
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5JTD0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

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