Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, HCT 116 cells, HeLa cells, K-562 cells, SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26563-1-AP targets TIRAP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24230 Product name: Recombinant human TIRAP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-235 aa of BC032474 Sequence: MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLSWHLLYHGTPEIGVK 相同性解析による交差性が予測される生物種 |
| Full Name | toll-interleukin 1 receptor (TIR) domain containing adaptor protein |
| Calculated molecular weight | 256 aa, 28 kDa |
| Observed molecular weight | 26-28 kDa |
| GenBank accession number | BC032474 |
| Gene Symbol | TIRAP |
| Gene ID (NCBI) | 114609 |
| RRID | AB_3669548 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P58753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIRAP, also known as MyD88-adaptor Like (MAL), is a crucial intracellular signaling molecule that plays a significant role in the regulation of immune responses, particularly in the context of Toll-like receptor (TLR)-mediated innate immune signaling. It functions as an adapter molecule that associates with receptor-mediated activation of host immune signaling. The innate immune system recognizes microbial pathogens through receptors, including TLRs, which identify pathogen-associated molecular patterns (PAMPs).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIRAP antibody 26563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
