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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of mouse small intestine using 19747-1-AP (same clone as 19747-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of mouse small intestine using 19747-1-AP (same clone as 19747-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of human kidney using 19747-1-AP (same clone as 19747-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    WB analysis of human placenta using 19747-1-AP (same clone as 19747-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    IHC staining of human colon using 19747-1-AP (same clone as 19747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TINAG-Specific Polyclonal antibody (19747-1-AP)

    IHC staining of human colon using 19747-1-AP (same clone as 19747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19747-1-PBS in a different storage buffer formulation.

TINAG-Specific Polyclonal antibody, PBS Only

TINAG-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19747-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19747-1-PBS targets TINAG-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name tubulointerstitial nephritis antigen
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_014464
Gene Symbol TINAG
Gene ID (NCBI) 27283
RRIDAB_10642433
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJW2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.

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