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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIMM44 Recombinant antibody (85134-5-RR)

    WB analysis using 85134-5-RR (same clone as 85134-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85134-5-RR (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using TIMM44 Recombinant antibody (85134-5-RR)

    WB analysis of Neuro-2a using 85134-5-RR (same clone as 85134-5-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 85134-5-RR (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TIMM44 Recombinant antibody (85134-5-RR)

    IF Staining of HepG2 using 85134-5-RR (same clone as 85134-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (85134-5-RR, Clone: 242774A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85134-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85134-5-RR

    Affinity and Kinetic Characterization of 85134-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85134-5-RR against Human TIMM44 were performed. The affinity constant is below 1 pM.

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TIMM44 Recombinant antibody, PBS Only

TIMM44 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85134-5-PBS
Clone No.242774A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TIMM 44, TIM44, MIMT44, 242774A4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85134-5-PBS targets TIMM44 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TIMM44 fusion protein Ag4834 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 44 homolog (yeast)
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight45 kDa
GenBank accession numberBC033628
Gene Symbol TIMM44
Gene ID (NCBI) 10469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43615
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).