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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IHC staining of human tonsillitis using 12008-1-AP (same clone as 12008-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IHC staining of human tonsillitis using 12008-1-AP (same clone as 12008-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IHC staining of human liver using 12008-1-AP (same clone as 12008-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IHC staining of human liver using 12008-1-AP (same clone as 12008-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIM4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IF Staining of human tonsillitis using 12008-1-AP (same clone as 12008-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIM4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TIM4 Polyclonal antibody (12008-1-AP)

    IF Staining of human tonsillitis using 12008-1-AP (same clone as 12008-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIM4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12008-1-PBS in a different storage buffer formulation.

TIM4 Polyclonal antibody, PBS Only

TIM4 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 12008-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

TIMD4, SMUCKLER, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12008-1-PBS targets TIM4 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2621

Product name: Recombinant human TIMD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-378 aa of BC008988

Sequence: SETVVTEVLGHRVTLPCLYSSWSHNSNSMCWGKDQCPYSGCKEALIRTDGMRVTSRKSAKYRLQGTIPRGDVSLTILNPSESDSGVYCCRIEVPGWFNDVKINVRLNLQRASTTTHRTATTTTRRTTTTSPTTTRQMTTTPAALPTTVVTTPDLTTGTPLQMTTIAVFTTANTCLSLTPSTLPEEATGLLTPEPSKEGPILTAESETVLPSDSWSSAESTSADTVLLTSKESKVWDLPSTSHVSMWKTSDSVSSPQPGASDTAVPEQNKTTKTGQMDGIPMSMKNEMPISQLLMIIAPSLGFVLFALFVAFLLRGKLMETYCSQKHTRLDYIGDSKNVLNDVQHGREDEDGLFTL

相同性解析による交差性が予測される生物種
Full Name T-cell immunoglobulin and mucin domain containing 4
Calculated molecular weight 378 aa, 42 kDa
GenBank accession numberBC008988
Gene Symbol TIM4
Gene ID (NCBI) 91937
RRIDAB_2877816
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96H15
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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