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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIM22 Polyclonal antibody (14927-1-AP)

    WB analysis using 14927-1-AP (same clone as 14927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14927-1-AP (TIM22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TIM22 Polyclonal antibody (14927-1-AP)

    IP experiment of mouse liver using 14927-1-AP (same clone as 14927-1-PBS)

    IP result of anti-TIM22 (IP:14927-1-AP, 4ug; Detection:14927-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIM22 Polyclonal antibody (14927-1-AP)

    IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TIM22 Polyclonal antibody (14927-1-AP)

    IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TIM22 Polyclonal antibody, PBS Only

TIM22 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14927-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14927-1-PBS targets TIM22 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6689

Product name: Recombinant human TIMM22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-194 aa of BC002324

Sequence: MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR

相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC002324
Gene Symbol TIM22
Gene ID (NCBI) 29928
RRIDAB_11183050
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y584
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.

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