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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tie1 Polyclonal antibody

Tie1 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 19329-1-AP

Synonyms

JTK14, TIE, TIE1

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 19329-1-AP

    WB analysis of K-562 using 19329-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 19329-1-AP

    WB analysis of NIH/3T3 using 19329-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (Tie1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 19329-1-AP

    WB analysis of MCF-7 using 19329-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HUVEC using 19329-1-AP

    FC experiment of HUVEC using 19329-1-AP

    1X10^6 HUVEC cells were stained with 0.2ug TIE1 antibody (19329-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, NIH/3T3 cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19329-1-AP is Tie1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tie1 fusion protein Ag4728 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Calculated molecular weight 1138 aa, 125 kDa
Observed molecular weight 154 kDa
GenBank accession numberBC038239
Gene symbol TIE1
Gene ID (NCBI) 7075
RRIDAB_10863655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Tie1 antibody 19329-1-APDownload protocol
FC protocol for Tie1 antibody 19329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols