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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TICAM2 Polyclonal antibody (19524-1-AP)

    WB analysis using 19524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TICAM2 Polyclonal antibody (19524-1-AP)

    WB analysis of PC-3 using 19524-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TICAM2 Polyclonal antibody (19524-1-AP)

    WB analysis of HeLa using 19524-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TICAM2 Polyclonal antibody (19524-1-AP)

    WB analysis of human testis using 19524-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TICAM2 Polyclonal antibody (19524-1-AP)

    IHC staining of mouse testis using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TICAM2 Polyclonal antibody (19524-1-AP)

    IHC staining of mouse brain using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using TICAM2 Polyclonal antibody (19524-1-AP)

    IHC staining of human prostate hyperplasia using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using TICAM2 Polyclonal antibody (19524-1-AP)

    IHC staining of human prostate hyperplasia using 19524-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TICAM2 Polyclonal antibody

TICAM2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19524-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, human testis tissue, PC-3 cells
Positive IHC detected inmouse testis tissue, human prostate hyperplasia tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19524-1-AP targets TICAM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name toll-like receptor adaptor molecule 2
Calculated molecular weight 27 kDa
Observed molecular weight 32 kDa
GenBank accession numberNM_021649
Gene Symbol TICAM2
Gene ID (NCBI) 353376
RRIDAB_10791887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32 kDa.

Protocols

Product Specific Protocols
WB protocol for TICAM2 antibody 19524-1-APDownload protocol
IHC protocol for TICAM2 antibody 19524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols