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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TIAM2 Polyclonal antibody (26870-1-AP)

    WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TIAM2 Polyclonal antibody (26870-1-AP)

    WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using TIAM2 Polyclonal antibody (26870-1-AP)

    IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using TIAM2 Polyclonal antibody (26870-1-AP)

    FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

TIAM2 Polyclonal antibody, PBS Only

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 26870-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26870-1-PBS targets TIAM2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25469

Product name: Recombinant human TIAM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1346-1701 aa of BC137275

Sequence: KDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS

相同性解析による交差性が予測される生物種
Full Name T-cell lymphoma invasion and metastasis 2
Observed molecular weight190-200 kDa
GenBank accession numberBC137275
Gene Symbol TIAM2
Gene ID (NCBI) 26230
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVF5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.

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