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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis using 19807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of HeLa using 19807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of mouse kidney using 19807-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    WB analysis of HepG2 using 19807-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human hepatocirrhosis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human testis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THUMPD3 Polyclonal antibody (19807-1-AP)

    IHC staining of human testis using 19807-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    IF Staining of HeLa using 19807-1-AP

    Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THUMPD3 Polyclonal antibody (19807-1-AP)

    IF Staining of HepG2 using 19807-1-AP

    Immunofluorescent analysis of HepG2 cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

THUMPD3 Polyclonal antibody

THUMPD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19807-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.1.1.256, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, L02 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19807-1-AP targets THUMPD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13833

Product name: Recombinant human THUMPD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 161-507 aa of BC001622

Sequence: GQEVKIDQRNVKKEFTSHALDSHILDYYENPAIKEDVSTLIGDDLASCKDETDESSKEETEPQVLKFRVTCNRAGEKHCFTSNEAARDFGGAVQDYFKWKADMTNFDVEVLLNIHDNEVIVGIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCDPLPYDIIVDPMCGTGAIPIEGATEWSDCFHIAGDNNPLAVNRAANNIASLLTKSQIKEGKPSWGLPIDAVQWDICNLPLRTGSVDIIVTDLPFGKRMGSKKRNWNLYPACLREMSRVCTPTTGRAVLLTQDTKCFTKALSGMRHVWRKVDTVWVNVGGLRAAVYVLIRTPQAFVHPSEQDGERGTLWQCKE

相同性解析による交差性が予測される生物種
Full Name THUMP domain containing 3
Calculated molecular weight 507 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC001622
Gene Symbol THUMPD3
Gene ID (NCBI) 25917
RRIDAB_10644140
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs.

Protocols

Product Specific Protocols
IF protocol for THUMPD3 antibody 19807-1-APDownload protocol
IHC protocol for THUMPD3 antibody 19807-1-APDownload protocol
WB protocol for THUMPD3 antibody 19807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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