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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THUMPD2 Polyclonal antibody (21054-1-AP)

    WB analysis using 21054-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21054-1-AP (THUMPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THUMPD2 Polyclonal antibody (21054-1-AP)

    IF Staining of HeLa using 21054-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THUMPD2 antibody (21054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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THUMPD2 Polyclonal antibody

THUMPD2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21054-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

THUMP domain-containing protein 2, THUMP domain containing 2, C2orf8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21054-1-AP targets THUMPD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THUMPD2 fusion protein Ag13736 相同性解析による交差性が予測される生物種
Full Name THUMP domain containing 2
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight56 kDa
GenBank accession numberBC004163
Gene Symbol THUMPD2
Gene ID (NCBI) 80745
RRIDAB_3085634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THUMP domain-containing protein 2 (THUMPD2) is the methyltransferase, which interacts with an auxiliary protein, TRMT112, to catalyze U6 m2G72 formation. Western blot analysis detected THUMPD2 at an apparent molecular mass of 56 kDa.

Protocols

Product Specific Protocols
WB protocol for THUMPD2 antibody 21054-1-APDownload protocol
IF protocol for THUMPD2 antibody 21054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols