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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THRAP3 Recombinant antibody (85093-2-RR)

    WB analysis using 85093-2-RR (same clone as 85093-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85093-2-RR (THRAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85093-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using THRAP3 Recombinant antibody (85093-2-RR)

    WB analysis of human brain using 85093-2-RR (same clone as 85093-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 85093-2-RR (THRAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85093-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THRAP3 Recombinant antibody (85093-2-RR)

    IF Staining of HeLa using 85093-2-RR (same clone as 85093-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THRAP3 antibody (85093-2-RR, Clone: 242268D8 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85093-2-PBS in a different storage buffer formulation.

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THRAP3 Recombinant antibody, PBS Only

THRAP3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85093-2-PBS
Clone No.242268D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85093-2-PBS targets THRAP3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor associated protein 3
Calculated molecular weight 109 kDa
Observed molecular weight109-130 kDa
GenBank accession numberNM_005119
Gene Symbol THRAP3
Gene ID (NCBI) 9967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2W1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation.