Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
19744-1-AP targets THRAP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | thyroid hormone receptor associated protein 3 |
| Calculated molecular weight | 109 kDa |
| Observed molecular weight | 109-130 kDa |
| GenBank accession number | NM_005119 |
| Gene Symbol | THRAP3 |
| Gene ID (NCBI) | 9967 |
| RRID | AB_11232035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2W1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IHC protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IF protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IP protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. | ||
J Cell Sci Novel stress granules-like structures are induced via a paracrine mechanism during viral infection. | ||
Cancer Res NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer | ||
Mol Cell Proteomics Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics | ||
Commun Biol NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing |