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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    IF Staining of U2OS using 83435-1-RR (same clone as 83435-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83435-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    IF Staining of U2OS using 83435-1-RR (same clone as 83435-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83435-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    FC experiment of U2OS using 83435-1-RR (same clone as 83435-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83435-1-PBS in a different storage buffer formulation.

THOC5 Recombinant monoclonal antibody, PBS Only

THOC5 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83435-1-PBS
Clone No.240013F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

KIAA0983, Functional spliceosome-associated protein 79, C22orf19, 240013F7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83435-1-PBS targets THOC5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6664

Product name: Recombinant human THOC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 354-683 aa of BC003615

Sequence: CKDDSVLHLTFYYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSASHMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTIAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGTAKLQAAVVLNPGYSSIPPIFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR

相同性解析による交差性が予測される生物種
Full Name THO complex 5
Calculated molecular weight 79 kDa
GenBank accession numberBC003615
Gene Symbol THOC5
Gene ID (NCBI) 8563
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13769
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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