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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of mouse brain using 14692-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of HepG2 using 14692-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of mouse skeletal muscle using 14692-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CTMP Polyclonal antibody (14692-1-AP)

    IP experiment of mouse brain using 14692-1-AP

    IP result of anti-CTMP (IP:14692-1-AP, 4ug; Detection:14692-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTMP Polyclonal antibody (14692-1-AP)

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTMP Polyclonal antibody (14692-1-AP)

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTMP Polyclonal antibody (14692-1-AP)

    IF Staining of HeLa using 14692-1-AP

    Immunofluorescent analysis of Hela cells, using CTMP antibody 14692-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CTMP Polyclonal antibody

CTMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14692-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

THEM4, EC:3.1.2.2, Carboxyl-terminal modulator protein, Acyl-coenzyme A thioesterase THEM4, Acyl-CoA thioesterase THEM4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14692-1-AP targets CTMP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6244

Product name: Recombinant human THEM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC065277

Sequence: MLRSCAARLRTLGALCRPPVGRRLPGSEPRPELRSFSSEEVILKDCSVPNPSWNKDLRLLFDQFMKKCEDGSWKRLPSYKRTPTEWIQDFKTHFLDPKLMKEEQMSQAQLFTRSFDDGLGFEYVMFYNDIEKRMVCLFQGGPYLEGPPGFIHGGAIATMIDATVGMCAMMAGGIVMTANLNINYKRPIPLCSVVMINSQLDKVEGRKFFVSCNVQSVDEKTLYSEATSLFIKLNPAKSLT

相同性解析による交差性が予測される生物種
Full Name thioesterase superfamily member 4
Calculated molecular weight 27 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC065277
Gene Symbol CTMP
Gene ID (NCBI) 117145
RRIDAB_2271685
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T1C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CTMP antibody 14692-1-APDownload protocol
IHC protocol for CTMP antibody 14692-1-APDownload protocol
IP protocol for CTMP antibody 14692-1-APDownload protocol
WB protocol for CTMP antibody 14692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Heliyon

PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by ubiquitinating CTMP and p21

Authors - Kai Sun
View Article
WB

FASEB J

Activation of SIK2 inhibits gluconeogenesis and alleviates lipogenesis-induced inflammatory response by SIK2-CRTC2-ACC1 in hepatocytes of large yellow croaker (Larimichthys crocea)

Authors - Shangzhe Han
View Article
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