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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTMP Polyclonal antibody

CTMP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14692-1-AP

Synonyms

CTMP, THEM4

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 14692-1-AP

    WB analysis of mouse brain using 14692-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14692-1-AP

    WB analysis of HepG2 using 14692-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 14692-1-AP

    WB analysis of mouse skeletal muscle using 14692-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14692-1-AP

    IP experiment of mouse brain using 14692-1-AP

    IP result of anti-CTMP (IP:14692-1-AP, 4ug; Detection:14692-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of human endometrial cancer using 14692-1-AP

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human endometrial cancer using 14692-1-AP

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 14692-1-AP

    IF Staining of HeLa using 14692-1-AP

    Immunofluorescent analysis of Hela cells, using CTMP antibody 14692-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14692-1-AP targets CTMP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTMP fusion protein Ag6244 相同性解析による交差性が予測される生物種
Full Name thioesterase superfamily member 4
Calculated molecular weight 27 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC065277
Gene symbol THEM4
Gene ID (NCBI) 117145
RRIDAB_2271685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CTMP antibody 14692-1-APDownload protocol
IHC protocol for CTMP antibody 14692-1-APDownload protocol
IF protocol for CTMP antibody 14692-1-APDownload protocol
IP protocol for CTMP antibody 14692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols