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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THEM2 Polyclonal antibody

THEM2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16782-1-AP

Synonyms

ACOT13, Acyl CoA thioesterase 13, HT012, PNAS 27, THEM2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 16782-1-AP

    WB analysis of HeLa using 16782-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human colon using 16782-1-AP

    WB analysis of human colon using 16782-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 16782-1-AP

    WB analysis of human liver using 16782-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 16782-1-AP

    WB analysis of human brain using 16782-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 16782-1-AP

    WB analysis of mouse kidney using 16782-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16782-1-AP

    WB analysis of HepG2 using 16782-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16782-1-AP

    IHC staining of human kidney using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16782-1-AP (THEM2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IHC staining of human kidney using 16782-1-AP

    IHC staining of human kidney using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16782-1-AP (THEM2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ACOT13/THEM2 antibody included
  • IF Staining of HepG2 using 16782-1-AP

    IF Staining of HepG2 using 16782-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16782-1-AP (THEM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, human colon tissue, human liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACOT13/THEM2 Ready-To-Use IHC Kit. ACOT13/THEM2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16782-1-AP targets THEM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THEM2 fusion protein Ag10212 相同性解析による交差性が予測される生物種
Full Name thioesterase superfamily member 2
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000894
Gene symbol THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for THEM2 antibody 16782-1-APDownload protocol
IHC protocol for THEM2 antibody 16782-1-APDownload protocol
IF protocol for THEM2 antibody 16782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols