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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THAP11 Recombinant monoclonal antibody (84373-6-RR)

    WB analysis using 84373-6-RR (same clone as 84373-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using THAP11 Recombinant monoclonal antibody (84373-6-RR)

    WB analysis of HepG2 using 84373-6-RR (same clone as 84373-6-PBS)

    WB result of THAP11 antibody (84373-6-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP11 transfected HepG2 cells. This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THAP11 Recombinant monoclonal antibody (84373-6-RR)

    IF Staining of HeLa using 84373-6-RR (same clone as 84373-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using THAP11 Recombinant monoclonal antibody (84373-6-RR)

    FC experiment of HepG2 using 84373-6-RR (same clone as 84373-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84373-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84373-6-RR

    Affinity and Kinetic Characterization of 84373-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

THAP11 Recombinant monoclonal antibody, PBS Only

THAP11 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84373-6-PBS
Clone No.241590E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

241590E5, CTG B43a, CTG B45d, HRIHFB2206, RONIN

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84373-6-PBS targets THAP11 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19342

Product name: Recombinant human THAP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC012182

Sequence: MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM

相同性解析による交差性が予測される生物種
Full Name THAP domain containing 11
Calculated molecular weight 314 aa, 34 kDa
Observed molecular weight45 kDa
GenBank accession numberBC012182
Gene Symbol THAP11
Gene ID (NCBI) 57215
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96EK4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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