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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of CHO cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of CHO cells using 12909-1-AP

    WB result of 12909-1-AP from Takashi Kobayashi.

  • Western Blot (WB) analysis of HeLa cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of HeLa using 12909-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human testis using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human testis using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of rat ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of rat ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    IF Staining of A549 using 12909-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

THADA Polyclonal antibody

THADA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12909-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

tRNA (32-2'-O)-methyltransferase regulator THADA, Thyroid adenoma-associated protein, thyroid adenoma associated, KIAA1767, GITA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, CHO cells
Positive IHC detected inhuman pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12909-1-AP targets THADA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3881

Product name: Recombinant human THADA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 59-400 aa of BC025773

Sequence: TSSALSMGPFVPFIMRCGHSPVYHSREMAARALVPFVMIDHIPNTIRSLLSTLPSCTDQCFRQNHIHGTLLQVFHLLQAYSDSKHGTNSDFQHELTDITVCTKAKLWLAKRQNPCLVTRAVYIDILFLLTCCLNRSAKDNQPVLESLGFWEEVRGIISGSELITGFPWAFKVPGLPQYLQSLTRLAIAAVWAAAAKSGERETNVPISFSQLLESAFPEVRSLTLEALLEKFLAAASGLGEKGVPPLLCNMGEKFLLLAMKENHPECFCKILKILHCMDPGEWLPQTEHCVHLTPKEFLIWTMDIASNERSEIQSVALRLASKVISHHMQTCVENRELIAAEL

相同性解析による交差性が予測される生物種
Full Name thyroid adenoma associated
Calculated molecular weight 220 kDa
Observed molecular weight 183 kDa
GenBank accession numberBC025773
Gene Symbol THADA
Gene ID (NCBI) 63892
RRIDAB_10638448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YHU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for THADA antibody 12909-1-APDownload protocol
IHC protocol for THADA antibody 12909-1-APDownload protocol
WB protocol for THADA antibody 12909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
  • KD Validated
View Article
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