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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    WB analysis using RMX00053

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00053 (TH antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    IHC staining of mouse brain using RMX00053

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00053 (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    IHC staining of mouse brain using RMX00053

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00053 (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    IHC staining of mouse brain using RMX00053

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00053 (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    IF Staining of mouse brain using RMX00053

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TH antibody (RMX00053) at dilution of 1:5000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Multi-rAb® TH Multi-Recombinant antibody (RMX00053)

    IF Staining of PC-12 using RMX00053

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (RMX00053) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® TH Multi-Recombinant antibody

TH Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00053

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00053 targets TH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name tyrosine hydroxylase
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight60 kDa
GenBank accession numberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
RRIDAB_3750105
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP07101
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.

Protocols

Product Specific Protocols
IF protocol for TH antibody RMX00053Download protocol
IHC protocol for TH antibody RMX00053Download protocol
WB protocol for TH antibody RMX00053Download protocol
Standard Protocols
Click here to view our Standard Protocols
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