Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 18 publications below |
| IHC | See 8 publications below |
| IF | See 15 publications below |
Product Information
66334-1-Ig targets TH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23075 Product name: Recombinant human TH protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-260 aa of BC104967 Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER 相同性解析による交差性が予測される生物種 |
| Full Name | tyrosine hydroxylase |
| Calculated molecular weight | 528 aa, 59 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC104967 |
| Gene Symbol | TH |
| Gene ID (NCBI) | 7054 |
| RRID | AB_2881714 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P07101 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TH antibody 66334-1-Ig | Download protocol |
| WB protocol for TH antibody 66334-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson's disease | ||
J Neuroinflammation Activation of HCA2 regulates microglial responses to alleviate neurodegeneration in LPS-induced in vivo and in vitro models | ||
J Transl Med Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson's disease progression by targeting DCLK1 | ||
NPJ Parkinsons Dis Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling | ||
Neural Regen Res Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson's disease. | ||
Antioxidants (Basel) Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency |