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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TH Polyclonal antibody (25859-1-AP)

    WB analysis using 25859-1-AP (same clone as 25859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IP experiment of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:20000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TH Polyclonal antibody (25859-1-AP)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TH Polyclonal antibody (25859-1-AP)

    IF Staining of rat brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, red). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using TH Polyclonal antibody (25859-1-AP)

    IF Staining of PC-12 using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

TH Polyclonal antibody, PBS Only

TH Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 25859-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25859-1-PBS targets TH in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23075

Product name: Recombinant human TH protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-260 aa of BC104967

Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

相同性解析による交差性が予測される生物種
Full Name tyrosine hydroxylase
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
RRIDAB_2716568
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07101
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

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