Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse testis using 15847-1-AP (same clone as 15847-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
WB analysis using 15847-1-AP (same clone as 15847-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 15847-1-AP (same clone as 15847-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15847-1-AP (same clone as 15847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15847-1-PBS in a different storage buffer formulation.

TGM4 Polyclonal antibody, PBS Only

TGM4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15847-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15847-1-PBS targets TGM4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8608 Product name: Recombinant human TGM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 335-684 aa of BC007003 Sequence: KRPDLPKGYDGWQAVDATSQERSQGVFCCGPSPLTAIRKGDIFIVYDTRFVFSEVNGDRLIWLVKMVNGQEELHVISMETTSIGKNISTKAVGQDRRRDITYEYKYPEGSSEERQVMDHAFLLLSSEREHRRPVKENFLHMSVQSDDVLLGNSVNFTVILKRKTAALQNVNILGSFELQLYTGKKMAKLCDLNKTSQIQGQVSEVTLTLDSKTYINSLAILDDEPVIRGFIIAEIVESKEIMASEVFTSFQYPEFSIELPNTGRIGQLLVCNCIFKNTLAIPLTDVKFSLESLGISSLQTSDHGTVQPGETIQSQIKCTPIKTGPKKFIVKLSSKQVKEINAQKIVLITK 相同性解析による交差性が予測される生物種
Full Name	transglutaminase 4 (prostate)
Calculated molecular weight	684 aa, 77 kDa
Observed molecular weight	68-70 kDa
GenBank accession number	BC007003
Gene Symbol	TGM4
Gene ID (NCBI)	7047
RRID	AB_2287328
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49221
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 15847-1-AP (same clone as 15847-1-PBS)

WB analysis using 15847-1-AP (same clone as 15847-1-PBS)

WB analysis of human testis using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human testis using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human testis using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human brain using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human brain using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human ovary using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human ovary using 15847-1-AP (same clone as 15847-1-PBS)

IHC staining of human kidney using 15847-1-AP (same clone as 15847-1-PBS)