Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15100-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15100-1-AP
WB result of TGM2 antibody (15100-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGM2 transfected HepG2 cells.
WB analysis of HUVEC using 15100-1-AP
HUVEC cells were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15100-1-AP
IP result of anti-TGM2 (IP:15100-1-AP, 4ug; Detection:15100-1-AP 1:400) with HeLa cells lysate 1200ug.
IHC staining of human liver cancer using 15100-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15100-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15100-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15100-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15100-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

Erythrocyte transglutaminase, TG(C), TG2, TGase 2, TGase C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (27)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HUVEC cells, HepG2 cells, BxPC-3 cells, HeLa cells, A549 cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 19 publications below
IHC	See 8 publications below
IF	See 9 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

15100-1-AP targets TGM2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7439 Product name: Recombinant human TGM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC003551 Sequence: MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQP 相同性解析による交差性が予測される生物種
Full Name	transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight	77 kDa
Observed molecular weight	77-80 kDa
GenBank accession number	BC003551
Gene Symbol	TGM2
Gene ID (NCBI)	7052
ENSEMBL Gene ID	ENSG00000198959
RRID	AB_2202885
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21980
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.

Protocols

Product Specific Protocols
IHC protocol for TGM2 antibody 15100-1-AP	Download protocol
IP protocol for TGM2 antibody 15100-1-AP	Download protocol
WB protocol for TGM2 antibody 15100-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nature Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease Authors - Yael Gruper View Article
human,canine	IF	Nat Genet New genes helped acorn barnacles adapt to a sessile lifestyle Authors - Zhaofang Han View Article
human	WB,IF	Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer Authors - Xingbin Hu View Article
human	WB,IF	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	WB	Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Authors - Chunqing Wang View Article
human	WB,IHC	Cell Oncol (Dordr) Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer Authors - Shan Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15100-1-AP

WB analysis of HepG2 using 15100-1-AP

WB analysis of HUVEC using 15100-1-AP

IP experiment of HeLa using 15100-1-AP

IHC staining of human liver cancer using 15100-1-AP

IHC staining of human liver cancer using 15100-1-AP

IHC staining of human liver cancer using 15100-1-AP

IHC staining of human liver cancer using 15100-1-AP

TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease

New genes helped acorn barnacles adapt to a sessile lifestyle

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer