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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    WB analysis using 83167-5-RR (same clone as 83167-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    IHC staining of human placenta using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    IHC staining of human placenta using 83167-5-RR (same clone as 83167-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    IF Staining of HeLa using 83167-5-RR (same clone as 83167-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGFB2-Specific antibody (83167-5-RR, Clone: 230461F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    FC experiment of A549 using 83167-5-RR (same clone as 83167-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tgfb2-Specific Recombinant Antibody (83167-5-RR, Clone:230461F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-87 MG cells using TGFB2/TGF-beta 2 Recombinant monoclonal antibody (83167-5-RR)

    FC experiment of U-87 MG using 83167-5-RR (same clone as 83167-5-PBS)

    1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug Tgfb2-Specific Recombinant Antibody (83167-5-RR, Clone:230461F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83167-5-PBS in a different storage buffer formulation.

TGFB2/TGF-beta 2 Recombinant monoclonal antibody, PBS Only

TGFB2/TGF-beta 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83167-5-PBS
Clone No.230461F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TGFB2-Specific, TGFB2, TGF-beta 2, 230461F5, Glioblastoma-derived T-cell suppressor factor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83167-5-PBS targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta 2
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_003238
Gene Symbol TGFB2
Gene ID (NCBI) 7042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP61812
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.

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