Validation Data Gallery

Filter: AllWBIF-PIF/ICC

WB analysis using 21898-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 21898-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 21898-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 21898-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21898-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21898-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21898-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of human stomach cancer using 21898-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 21898-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

TGF beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 21898-1-AP

Publications(951)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Synonyms

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (951)

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skin tissue, A549 cells, HeLa cells, K-562 cells, L-929 cells, mouse spleen tissue, rat skin tissue
Positive IF-P detected in	human stomach cancer tissue
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 703 publications below
IHC	See 211 publications below
IF	See 103 publications below
IP	See 2 publications below
ELISA	See 2 publications below
CoIP	See 1 publications below

Product Information

21898-1-AP targets TGF Beta 1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, zebrafish, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13591 Product name: Recombinant human TGFB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 14-258 aa of BC000125 Sequence: PLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFL 相同性解析による交差性が予測される生物種
Full Name	transforming growth factor, beta 1
Calculated molecular weight	44 kDa
Observed molecular weight	44 kDa
GenBank accession number	BC000125
Gene Symbol	TGFB1
Gene ID (NCBI)	7040
RRID	AB_2811115
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01137
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

Publications

Species	Application	Title
mouse	IHC	Signal Transduct Target Ther Integrative analysis of non-small cell lung cancer identifies Jumonji domain-containing 6/ETS homologous factor axis as a target to overcome radioresistance Authors - Manni Wang View Article
human	IHC	Signal Transduct Target Ther COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy Authors - Pei-Pei Zhang View Article
	WB	Signal Transduct Target Ther 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis Authors - Fu Peng View Article
human	WB	Cancer Cell Antiandrogen treatment induces stromal cell reprogramming to promote castration resistance in prostate cancer Authors - Hanling Wang View Article
human	WB,IF	Adv Mater Engineered Surfaces that Promote Capture of Latent Proteins to Facilitate Integrin-Mediated Mechanical Activation of Growth Factors Authors - Udesh Dhawan View Article
	IHC	Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine Authors - Peng Lou View Article

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Validation Data Gallery

WB analysis using 21898-1-AP

WB analysis of mouse skin using 21898-1-AP

WB analysis using 21898-1-AP

WB analysis using 21898-1-AP

WB analysis of K-562 using 21898-1-AP

WB analysis of HeLa using 21898-1-AP

WB analysis of A549 using 21898-1-AP

IF Staining of human stomach cancer using 21898-1-AP

IF Staining of HEK-293 using 21898-1-AP

TGF beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

Integrative analysis of non-small cell lung cancer identifies Jumonji domain-containing 6/ETS homologous factor axis as a target to overcome radioresistance

COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy

2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis

Antiandrogen treatment induces stromal cell reprogramming to promote castration resistance in prostate cancer

Engineered Surfaces that Promote Capture of Latent Proteins to Facilitate Integrin-Mediated Mechanical Activation of Growth Factors

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Able^™

Validation Data Gallery

WB analysis using 21898-1-AP

WB analysis of mouse skin using 21898-1-AP

WB analysis using 21898-1-AP

WB analysis using 21898-1-AP

WB analysis of K-562 using 21898-1-AP

WB analysis of HeLa using 21898-1-AP

WB analysis of A549 using 21898-1-AP

IF Staining of human stomach cancer using 21898-1-AP

IF Staining of HEK-293 using 21898-1-AP

TGF beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

Integrative analysis of non-small cell lung cancer identifies Jumonji domain-containing 6/ETS homologous factor axis as a target to overcome radioresistance

COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy

2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis

Antiandrogen treatment induces stromal cell reprogramming to promote castration resistance in prostate cancer

Engineered Surfaces that Promote Capture of Latent Proteins to Facilitate Integrin-Mediated Mechanical Activation of Growth Factors

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Able™

Able^™