- Featured Product
- KD/KO Validated
TGF Beta 1 Polyclonal antibody
TGF Beta 1 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA
Conjugate
Unconjugated
543
Cat no : 21898-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skin tissue, K-562 cells, HeLa cells, A549 cells, rat skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IF detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 427 publications below |
| IHC | See 124 publications below |
| IF | See 54 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
21898-1-AP targets TGF Beta 1 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rabbit, zebrafish, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGF Beta 1 fusion protein Ag13591 相同性解析による交差性が予測される生物種 |
| Full Name | transforming growth factor, beta 1 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC000125 |
| Gene symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| RRID | AB_2811115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).
This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| IF protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| IP protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Acta Pharm Sin B Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Autophagy Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andrea (Verified Customer) (03-22-2024) | We had a good confocal signal and beautiful bands in our experimental group of high TGFb
|
FH Kenzo (Verified Customer) (08-15-2023) | This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.
|
FH Celina (Verified Customer) (07-31-2023) | antibody worked well for IF of human cardiac ventricular fibroblasts
|
FH Udesh (Verified Customer) (12-06-2022) | The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.
 |
FH Yasuyo (Verified Customer) (05-24-2022) | Works great!!!
 |
FH P (Verified Customer) (12-01-2021) | Excellent
 |
FH Iram (Verified Customer) (09-05-2020) | Very clean bands for western
|
FH Qingyuan (Verified Customer) (11-05-2019) | The antibody clearly shows one band with the right molecular weight size.
|
