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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of K-562 using 10097-2-AP (same clone as 10097-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of HL-60 using 10097-2-AP (same clone as 10097-2-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of mouse liver using 10097-2-AP (same clone as 10097-2-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of PC-3 using 10097-2-AP (same clone as 10097-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of mouse liver using 10097-2-AP (same clone as 10097-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of human brain using 10097-2-AP (same clone as 10097-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of human brain using 10097-2-AP (same clone as 10097-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

TFPT Polyclonal antibody, PBS Only

TFPT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10097-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10097-2-PBS targets TFPT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0131

Product name: Recombinant human TFPT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-253 aa of BC001728

Sequence: SELETEVEFVSGGLGGSGLRERDEEEEAARGRRRRQRELNRRKYQALGRRCREIEQVNERVLNRLHQVQRITRRLQQERRFLMRVLDSYGDDYRASQFTIVLEDEGSQGTDAPTPGNAENEPPEKETLSPPRRTPAPPEPGSPAPGEGPSGRKRRRVPRDGRRAGNALTPELAPVQIKVEEDFGFEADEALDSSWVSRGPDKLLPYPTLASPAS

相同性解析による交差性が予測される生物種
Full Name TCF3 (E2A) fusion partner (in childhood Leukemia)
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC001728
Gene Symbol TFPT
Gene ID (NCBI) 29844
RRIDAB_2240572
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C1Z6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

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