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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TFPI Recombinant antibody (83838-5-RR)

    WB analysis of HepG2 using 83838-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83838-5-RR (TFPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TFPI Recombinant antibody (83838-5-RR)

    IF Staining of HepG2 using 83838-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (83838-5-RR, Clone: 240977B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using TFPI Recombinant antibody (83838-5-RR)

    FC experiment of HepG2 using 83838-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HUVEC cells using TFPI Recombinant antibody (83838-5-RR)

    FC experiment of HUVEC using 83838-5-RR

    1x10^6 HUVEC cells were surface stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 83838-5-RR

    Affinity and Kinetic Characterization of 83838-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83838-5-RR against Human TFPI were performed. The affinity constant is below 1 pM.

TFPI Recombinant monoclonal antibody

TFPI Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83838-5-RR
Clone No.240977B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240977B1, EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83838-5-RR targets TFPI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0855

Product name: Recombinant Human TFPI protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 29-282 aa of NM_006287.6

Sequence: DSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQRISKGGLIK

相同性解析による交差性が予測される生物種
Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Calculated molecular weight35 kDa
Observed molecular weight 40 kDa
GenBank accession numberNM_006287.6
Gene Symbol TFPI
Gene ID (NCBI) 7035
RRIDAB_3671421
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP10646-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Protocols

Product Specific Protocols
IF protocol for TFPI antibody 83838-5-RRDownload protocol
WB protocol for TFPI antibody 83838-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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