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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of human brain tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of human brain using 66114-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of HeLa using 66114-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of human testis using 66114-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human breast cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human prostate cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human prostate cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human liver cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human liver cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human breast cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFIP11 Monoclonal antibody (66114-1-Ig)

    IF Staining of HeLa using 66114-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TFIP11 Monoclonal antibody

TFIP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66114-1-Ig
Clone No.1A1C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1A1C8, bK445C9.6, hNtr1, HSPC006, NTR1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, human testis tissue, human brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66114-1-Ig targets TFIP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5821

Product name: Recombinant human TFIP11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 492-837 aa of BC033080

Sequence: IVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQHMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSVKDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVPMNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK

相同性解析による交差性が予測される生物種
Full Name tuftelin interacting protein 11
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_2881513
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

Protocols

Product Specific Protocols
WB protocol for TFIP11 antibody 66114-1-IgDownload protocol
IHC protocol for TFIP11 antibody 66114-1-IgDownload protocol
IF protocol for TFIP11 antibody 66114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols