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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of HeLa using 14436-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of K-562 using 14436-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of MCF-7 using 14436-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TFIP11 Polyclonal antibody (14436-1-AP)

    WB analysis of mouse testis using 14436-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFIP11 Polyclonal antibody (14436-1-AP)

    IF Staining of HeLa using 14436-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14436-1-AP (TFIP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TFIP11 Polyclonal antibody

TFIP11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14436-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

STIP, Septin and tuftelin-interacting protein 1, NTR1, HSPC006, hNtr1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14436-1-AP targets TFIP11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5821

Product name: Recombinant human TFIP11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 492-837 aa of BC033080

Sequence: IVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQHMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSVKDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVPMNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK

相同性解析による交差性が予測される生物種
Full Name tuftelin interacting protein 11
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_11182269
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

Protocols

Product Specific Protocols
IF protocol for TFIP11 antibody 14436-1-APDownload protocol
WB protocol for TFIP11 antibody 14436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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