Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
11596-1-PBS targets TFIIE Beta in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2136 Product name: Recombinant human TFIIE beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-291 aa of BC030572 Sequence: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK 相同性解析による交差性が予測される生物種 |
| Full Name | general transcription factor IIE, polypeptide 2, beta 34kDa |
| Calculated molecular weight | 291 aa, 33 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC030572 |
| Gene Symbol | GTF2E2 |
| Gene ID (NCBI) | 2961 |
| RRID | AB_2232722 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29084 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).