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Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using TFF1 Recombinant antibody (83467-2-RR)

    FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83467-2-RR

    Affinity and Kinetic Characterization of 83467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

TFF1 Recombinant monoclonal antibody, PBS Only

TFF1 Uni-rAb® Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83467-2-PBS
Clone No.240424F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240424F5, Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83467-2-PBS targets TFF1 in FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4541

Product name: Recombinant human TFF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-84 aa of BC032811

Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF

相同性解析による交差性が予測される生物種
Full Name trefoil factor 1
Calculated molecular weight 84 aa, 9 kDa
GenBank accession numberBC032811
Gene Symbol TFF1
Gene ID (NCBI) 7031
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04155
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.