Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 13734-1-AP (same clone as 13734-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13734-1-AP (TFF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 13734-1-AP (same clone as 13734-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (TFF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of rat stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IHC staining of rat stomach using 13734-1-AP (same clone as 13734-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 13734-1-AP (same clone as 13734-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13734-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TFF1 Polyclonal antibody, PBS Only

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13734-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13734-1-PBS targets TFF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4541 Product name: Recombinant human TFF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC032811 Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF 相同性解析による交差性が予測される生物種
Full Name	trefoil factor 1
Calculated molecular weight	84 aa, 9 kDa
Observed molecular weight	9-12 kDa
GenBank accession number	BC032811
Gene Symbol	TFF1
Gene ID (NCBI)	7031
RRID	AB_2202853
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04155
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13734-1-AP (same clone as 13734-1-PBS)

WB analysis of human kidney using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human stomach using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human colon cancer using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human ovary tumor using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of human stomach cancer using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of mouse stomach using 13734-1-AP (same clone as 13734-1-PBS)

IHC staining of rat stomach using 13734-1-AP (same clone as 13734-1-PBS)