Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13372-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13372-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13372-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13372-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13372-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 13372-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13372-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 13372-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 13372-1-AP
IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.
IHC staining of human renal cell carcinoma using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13372-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13372-1-AP

Publications(218)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay

Synonyms

Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (218)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human renal cell carcinoma tissue, rat spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 28 publications below
WB	See 179 publications below
IHC	See 20 publications below
IF	See 99 publications below
IP	See 6 publications below
CoIP	See 2 publications below

Product Information

13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TFEB fusion protein Ag4194 相同性解析による交差性が予測される生物種
Full Name	transcription factor EB
Calculated molecular weight	476 aa, 53 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC032448
Gene Symbol	TFEB
Gene ID (NCBI)	7942
RRID	AB_2199611
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19484
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Protocols

Product Specific Protocols
WB protocol for TFEB antibody 13372-1-AP	Download protocol
IHC protocol for TFEB antibody 13372-1-AP	Download protocol
IF protocol for TFEB antibody 13372-1-AP	Download protocol
IP protocol for TFEB antibody 13372-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	ACS Nano A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease. Authors - Jingyi Liu View Article
mouse	IF	Acta Neuropathol Wolframin is a novel regulator of tau pathology and neurodegeneration. Authors - Shuo Chen View Article
mouse	WB,IP,IF	Mol Cell Itaconate is a lysosomal inducer that promotes antibacterial innate immunity. Authors - Zhenxing Zhang View Article
mouse	WB,IF	J Hazard Mater VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes Authors - Tao Wang View Article
human	WB,IF	Autophagy SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis Authors - Qin Xia View Article
human	WB	Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration Authors - Huaizhen Liang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13372-1-AP

WB analysis of Jurkat using 13372-1-AP

WB analysis using 13372-1-AP

WB analysis of Jurkat using 13372-1-AP

WB analysis of HeLa using 13372-1-AP

WB analysis of human placenta using 13372-1-AP

WB analysis of NIH/3T3 using 13372-1-AP

WB analysis of rat brain using 13372-1-AP

IP experiment of Raji using 13372-1-AP

IHC staining of human renal cell carcinoma using 13372-1-AP

IHC staining of human renal cell carcinoma using 13372-1-AP

IHC staining of rat spleen using 13372-1-AP

IHC staining of rat spleen using 13372-1-AP

IF Staining of HeLa using 13372-1-AP

TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration