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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFDP2 Polyclonal antibody

TFDP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11500-1-AP

Synonyms

Dp 2, DP2, E2F dimerization partner 2, TFDP2, Transcription factor Dp 2

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 11500-1-AP

    WB analysis of mouse heart using 11500-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 11500-1-AP

    WB analysis of human brain using 11500-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 11500-1-AP

    WB analysis of human brain using 11500-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 11500-1-AP

    IP experiment of SH-SY5Y using 11500-1-AP

    IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:400) with SH-SY5Y cells lysate 1600ug.

Tested Applications

Positive WB detected inmouse heart tissue, human brain tissue
Positive IP detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

11500-1-AP targets TFDP2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFDP2 fusion protein Ag2054 相同性解析による交差性が予測される生物種
Full Name transcription factor Dp-2 (E2F dimerization partner 2)
Calculated molecular weight 446 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC021113
Gene symbol TFDP2
Gene ID (NCBI) 7029
RRIDAB_2202685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].

Protocols

Product Specific Protocols
WB protocol for TFDP2 antibody 11500-1-APDownload protocol
IP protocol for TFDP2 antibody 11500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Syst Biol

The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.

Authors - Caron Etienne E
View Article